The ADCC assay is a robust in vitro co-culture model used to validate potential therapeutic antibodies for their efficiency to mediate effector cell driven target cell cytotoxicity. Mimicking the natural immune response, the assay employs effector cells (PBMCs, NK cells, or Jurkat cells) to recognise and engage antibody bound target tumour cells. https://cellomaticsbio.com/adcc-assay-schematic/